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It can be a tricky part of the paper to write, so many scientists and researchers prefer to write it last, to make sure they haven’t missed anything important.
After all, a weakness in your paper might later inspire another research question, so be very clear about your assumptions early on.
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As long as you warn the reader about this, so that they are aware of the shortcomings, then they can easily judge the validity of the research for themselves.
This is much better than making them wait until you point the weaknesses out in the discussion.
In the introduction, you are attempting to inform the reader about the rationale behind the work, and to justify why your work is essential in the field.
The introduction does not have a strict word limit, unlike the abstract, but it should be as concise as possible.
The introduction serves a few different functions: it presents your topic, clarifies the context of the paper, attracts the attention of readers, and presents a thesis statement which will be explained and developed throughout the whole research. Explain what you’re going to focus on, and what questions you will answer.
The introduction also must grab attention and motivate your readers to keep reading.
The introduction is the place to highlight any weaknesses in the experiment from the start.
For example, an ideal experiment should have perfectly randomized samples, but there are many good reasons why this is not always possible.